MAKLER COUNTING CHAMBER PDF

Sperm count performed from undiluted specimen. No additional factors are necessary for calculation. Optimal depth: The depth of 10 microns eliminates blurring and allows sperm to move freely. The applied sample is observed in one focal plane. Built-in grid: The grid is on the cover glass.

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Constructed from two pieces of optically flat glass, the upper layer serves as a cover glass, with a 1 sq. Spacing is firmly secured by four quartz pins. Analysis Technique: A small, uncalibrated drop from a well mixed undiluted specimen is placed in the center of the Chamber by means of a simple rod and immediately covered.

A microscopic objective of x20 is required. Motility Evaluation: Non-motile sperm are counted within an area of nine or sixteen squares in the center of the grid. Moving sperms are then counted, and graded if desired. The procedure is repeated in several areas. Percentage of motility and its quality are then calculated. A drop of the immobilized specimen is then placed in the Chamber and counting initiated: sperm heads within a ten square area are counted in the same manner as blood cells are counted in a hemocytomer, their number represents their concentration in millions per ml.

In cases of oligospermic semen, sperms in the entire grid area are to be counted, representing their concentration in hundreds of thousands. The Chamber is easily rinsed with water for reuse. Contact surfaces are wiped with special lens paper after washing. Advantages: Applied spermatozoa are uniformly distributed and monolayered, and are observed in one focal plane.

Dilution is unnecessary even with concentrated specimens. Analysis is done directly from original specimen in its natural environment. All spermatozoa acquire friction free, horizontal movement and are always examined under constant conditions.

The specimen can be analyzed quickly as an office procedure while the patient is waiting, and even by an inexperienced person. Accuracy of analysis is enhanced through the elimination of the various steps required by the usual hemocytometric technique.

In addition, the fact that sperm motility is examined each time under identical conditions further increases accuracy. Errors incurred by uncontrolled pressure applied to the cover slip are thus avoided. The 10 micron depth of the Makler Chamber is ideal for still or movie camera photomicrography, as it approximately matches the field depth of the objective used in semen analysis. The Chamber is quickly and easily available for reuse.

In a busy laboratory a large number of tests per hour can be made by a single technician with minimal technical and material requirements. References: Makler, A.

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The volume of the squares counted is the one shown in the table at the top, depending on the size see figure on the right. The number of cells counted is the sum of all cells counted across squares in one chamber. The proportion of the cells counted applies if not all inner squares within a set square are counted i. The parts of the hemocytometer as viewed from the side are identified. For most applications, the four large corner squares are only used. The cells that are on or touching the top and left lines are counted, but the ones on or touching the right or bottom lines are ignored.

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